Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification
نویسندگان
چکیده
منابع مشابه
Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification
BACKGROUND Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and prevention of disease spread. An easy to perform and highly sensitive method is needed for routine implementation especially in the resource-limited rural healthcare settings where dengue is endemic. METHODS A single-tube reverse transcription-loop-media...
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Dengue is usually diagnosed by isolation of the virus, serology or molecular diagnostic methods. Several commercial kits for the diagnosis of dengue are existing, but concerns have arisen regarding to the affordability and performance characteristics of these kits. Hence, the loop-mediated isothermal amplification (LAMP) is potentially ideal to be used especially in resource limited environment...
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In recent years, researchers have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) test as a sensitive and specific technique. But the results of these studies were conflicting. The aim of this meta-analysis was to assess the accuracy of RT-LAMP technique for detecting dengue virus. We systematically searched PUBMED, EMBASE, Web of Science, and the Cochrane Lib...
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ژورنال
عنوان ژورنال: BMC Infectious Diseases
سال: 2013
ISSN: 1471-2334
DOI: 10.1186/1471-2334-13-387